You were assigned to work on a novel anticancer drug that will be used against pancreatic cancer; which cell lines, A549, HEK293, or Hela, would you choose for testing the anti-cancer drug? Explain why?

Answer these questions: 1. You were assigned to work on a novel anticancer drug that will be used against pancreatic cancer; which cell lines, A549, HEK293, or Hela, would you choose for testing the anti-cancer drug? Explain why? Suggest an alternative cell line and justify your answer if none of these lines are useful and provide a reference paper. 200 words max.2. You were given a vial containing 2 million viable Hela cells; after growing the cells for 48 hours, you noticed 90% were rounded and dead. What are the immediate parameters you need to check? 50 words max3. You started counting the cells on a hemacytometer under a microscope and saw that most cells are in clumps. Discuss why the cells are still in clumps. How can you resolve such an issue? 50 words max4. Given a trypsinized plate and cells collected in a 4.5 ml final volume, when 4 squares were counted using the hemacytometer, the average was 55 cells/square. Given that the counted sample was diluted by 1/2, how many cells do you have in total? How would you achieve seeding 500,000 cells per well (each well contains 2 ml of media)? 50 words max

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